Analysis of Andrographis Paniculata (Kalmegh) Extracts Through Hptlc

Abstract

Andrographis paniculata important bioactive constituent is andrographolide, mostly present in the leaves of plant and isolation procedure is easy from crude plant, present in form of crystalline solid. The geographical abundance of plant is Bengal and constituents consisting of lactones and flavones.

Aim: The quantitative determination of extracts of Andrographis paniculata (Kalmegh) by the use of High-Performance Thin Layer Chromatography method for determining of phytochemical compounds and FTIR for characterization.

Material and methods: A. paniculata fresh leaves were collected and dried; and grounded to a powdered form with the help of milling machine. The evaluation of extracts was done by calculating the % extraction yield, Lost on Drying, ash content, Chemical identification, Phytochemical screening of the extract like alkaloid, saponin, glycoside, phytosterol, terpinoid, tannis, flavanoids, anthraquinone derivatives. Further analytical method development for AGL(Aandrographolide) was done by High-Performance Thin Layer Chromatography (HPTLC) and Fourier-transform infrared spectroscopy (FTIR) studies were done for extract of Andrographolide.

Results and discussion: The % yield of extract was higher in 95 % ethanol (v/v) as the yield of A. paniculata in ethanolic extract of 95%, 70%, and 50%, were 23.6, 9.62 and 6.56 gm respectively Loss on drying result of Andrographis paniculata is not more than 10% and ash content is 7.63. Alkaloids, Flavonoids, Saponin, Terpenoid, Tannin, Glycosides, Phytosterol, and Proteins, were present and absence of Anthraquinone derivatives according to common phytochemical methods.