Evaluation Of Quorum Sening Lasr And Lasa Genes In Pseudomonus Aeruginosa And It`S Relation With Biofilm Formation

Abstract

100 clinical samples in total were gathered from various clinical sources. , from different ages   , the results of culture and biochemical tests showed that 65 isolate was P. aeruginosa. The source of these isolates which was burn swab n=22 (44%) ,urine culture n=11(22%) ,wound swab n=5(10%), sputum n=4(8%) ,ear swab, n=7(14%) and blood n=1(2%). Clinical specimen were collected through the period extending from September   2021 to January 2022, from Al-Yarmouk Teaching hospital and AL-Imameen Al-Kazmeen medical city.

 In addition, various classical and modern culturing with biochemical tests were conducted to differentiate these isolates from another nosocomial infection bacterium. On the other hand the study was also aimed to identify existence, dispersion and occurrence rate of Las A and Las R genes by conventional PCR technique, and this Genes believed that it have arelation In regards to the capacity of isolates to produce biofilms using the microtiter plate method (MTP), Bacteria exhibit two social phenomena: quorum sensing and biofilm development. The study of the relationship between quorum sensing and biofilms is known as sociomicrobiology.  

 The results showed that76% of isolates was biofilm producers on the other hand LasR Gene was revealed in 58% of the samples while LasA Gene was estimated in 56% in p.aeruginosa isolates. and these gene especially Las R was found in most producer biofilm isolates. The presence of the investigated Genes and the isolates' capacity to produce biofilms were significantly correlated.