Comparison of the effect of Aloe vera nanofibers enriched samples with Arnebia euchroma extracts with other treatment and a quantitative study of collagen І and collagen ІІІ genes expression in the skin of mice after micro-needling

Abstract

Background:  Mechanical injuries and aging, causes changes in skin collagen.  In the experimental and traditional methods, herbs and aloe vera gel are used to repair and rejuvenation of skin.

Objective:  In this study, the effect of and aloe vera nanofibers and their enriched nanofibers with extract (Arnebia euchorma) and other treatments were investigated and compared on the expression of collagen  І and collagen  ІІІ genes  in the skin mice after micro needling.

Methods: The experiments were performed under standard conditions. Micro needling was performed on each tested mouse and each was treated for 48 hours. expression of collagen  І and collagen  ІІІ  genes  was evaluated  by RT- PCR method. According to the obtained results, with treatments, Extract, gels, nanofibers and enriched nanofibers has a significant increase compared to the model group.

Resules:  Micro needling stimulated the skin and it allows to penetrate the skin by creating very small pores. These materials were also gradually released from nanofibers. Shikonin probebly inhibited the pathway NF-κB. Glucomannan, increases collagen  І and collagen  ІІІ genes  expression at the injury site by affecting  the MAP ERK pathway.

Conclusion: The process of expressing collagen  І and collagen ІІІ  gene is essential in repairing and rejuvenating   the skin.